To identify differentiation of nEPS for applying to any disease, we differentiated the nEPS into three germ layer cells of the ectoderm, the mesoderm, and the endoderm. Ectodermal cells formed neuronal cells, mesodermal cells formed adipocyte, osteoblast and chondrocyte, and endoderm cell led to hepatocyte and pancreatic beta cells.
In the field of bioengineering, active research was conducted on applying stem cells in tissue engineering and differentiation mechanism of pluripotent stem cells like embryonic stem cell and induced pluripotent stem cell, and multipotent adult stem cells like bone marrow-derived stromal cell and adipose tissue-derived stem cell.
By the discovery of the differentiation potency of stem cell into diverse cells, there have been studies on signaling mechanisms and differentiation mechanisms that induce differentiation of stem cells into specific cells. There is also a tissue engineering attempt to create 3D tissues using stem cell. Stem Cell Treatment and Research Institute (STRI) has been studying the differentiation of pluripotent stem cell derived from the umbilical cord into various body organ cells, as well as studying about new substances, proteins, culture environments and technologies in which such differentiation can occur more properly.
When nEPS cell was differentiated into the ectodermal cell, it was differentiated into shape of a nerve cell. Differentiation was verified by dyeing the cell with Nestin, which is only expressed in nerve cells. We were able to find that Nestin μm And Hoechst merge differentiated into a nerve cell. (Below – Neurocyte)
When nEPS cell was differentiated into liver cell as the endodermal cell, shape of liver cell was changed. Differentiation was verified by dyeing the cell with α-fetrotein, which is only expressed in liver cells. We were able to confirm that the α-fetrotein Hoechst merge differentiated into a liver cell. (Below – Hepatocyte)
When nEPS cell was differentiated into pancreatic B-cell, shape of pancreatic B-cell was changed. (Below - Pancreatic β-cell)
When nEPS cell was differentiated into kidney cell, shape of kidney cell was changed. (Below – kidney cell)
nEPS cell was differentiated into cartilage cell and osteoblast as mesodermal cells. Cartilage cell consists of collagen and Polysaccharide polymer substance.
Differentiation into cartilage cell was verified by dyeing the cell with Alcian blue, which represents blue color through combination with polysaccharide having carboxyl sulfate unit at pH of 2.5.
When nEPS cell was differentiated into T cell, the shape of T cell was changed. (Below – T cell)
When nEPS cell was differentiated into hematopoietic cell, shape of hematopoietic cell was changed. (Below – hematopoietic cell)
When nEPS cell was differentiated into fat cell, shape of fat cell was changed. (Below – fat cell)