– STRI was confirmed to differentiation of podocyte from nEPS.
– This discovery could enable STRI to move a step closer to make mini kidney using 3D podocyte speroid for research of organoid.
Kidney Disease program
Kidney diseases are currently a global public health problem, with an incidence that has reached epidemic proportions and continues to climb in the world wide. These trends correlate with the global rise in the aged population and the increasing prevalence of conditions that cause renal complications, namely cardiovascular disease, hypertension, and diabetes. Research in regenerative medicine, with the ultimate aim of generating functional replacement kidney tissue, offers the potential for new therapeutic strategies to treat kidney disease.
We have tried to differentiate kidney cells which have a rapid, efficient, and highly reproducible system to induce intermediate mesoderm cells from nEPS under precise, chemically defined, monolayer and 3D spheroid culture conditions.
We can make 3D spheroid of nEPS and then induce mesodermal cell for differentiating of podocyte. In a mature differentiated state, human podocytes express proteins such as WT1, nephrin, synaptopodin, and podocin.
The nEPS can be differentiated into podocyte cell and nEPS-derived podocyte expressed nephrin protein.
The progression of glomerular disorders leading to fibrosis and loss of function is dependent on the severity of podocyte injury and the capacity for tissue regeneration and podocyte replacement.
We should develop nEPS to nephron and renal tubule forming which is core structure in kidney tissue and test differentiation into kidney precursor cells.
The establishment of this system will facilitate and improve the direct differentiation of nEPS into cells of the kidney lineage for the purposes of bioengineering kidney tissue and nEPS cell disease modeling.